钙调素拮抗剂TFP对BGC-823细胞增殖的影响及其分子机制的探讨

EFFECT OF TFP, A INHIBITOR OF CALMODULIN, ON CELL PROLIFERATION IN BGC-823 CELLS AND IT'S MOLECULAR MECHANISM

为了进一步探讨钙调素拮抗剂 TFP对人胃癌细胞增殖的影响及其分子机制 ,利用MTT法及流式细胞光度术检测了 TFP对细胞增殖的影响 ,进一步利用 western blot的方法对细胞中 p1 6INK 4a和 cyclin D的表达水平进行了检测 .结果表明 :TFP处理可以明显提高 BGC- 82 3细胞中 p1 6INK 4a的表达水平 ,而 cyclin D1的水平则明显下降 ,提示 TFP可能通过影响 p1 6IN K4a的表达水平抑制细胞的增殖 .

To investigate the inhibition mechanism of calmodulin inhibi to r TFP in the proliferation of BGC-823 cells, the effect of TFP on the prolifera tion of BGC-823 and the level of p16INK4a and cyclinD are assayed respect ively by MTT, FCM and western blot. These results show that TFP inhibits the pro liferation of BGC-823 cells. The level of p16INK4a increases great ly. On the contrary, the level of cyclin D1 decreases obviously. This may be on e of the melecular mechanisms which is involved in the regulation of proliferati on in BGC-823 cells by TFP.