建立裸小鼠大肠癌肝脏微转移模型的方法研究

Establishment of the model of hepatic micrometastasis of colorectal cancer in nude rat

目的 :研究一种建立裸小鼠大肠癌肝脏微转移模型的方法。方法 :运用盲肠造疝原位瘤块接种法建立裸小鼠的大肠癌模型 ,同时以Alu基因为标志 ,利用PCR法检测接种后不同时期的裸小鼠肝脏中发生微转移的肿瘤细胞。结果 :盲肠原位接种瘤块后 2周 ,大部分裸小鼠均长出直径 1cm的盲肠实体瘤 ,接种成功率为 88%。接种后 4～ 6周的裸小鼠中 ,常规病理检查未发现异常 ,5 7.1% ( 4 7)肝组织中检测到Alu基因 ,表明裸小鼠肝脏中存在着微转移的肿瘤细胞。结论 :利用盲肠造疝瘤块原位接种法建立裸小鼠的大肠癌模型 ,方法简单且成功率高 ,同时易于观察肿瘤的生长。

Objective:To study on the method of building up the model of hepatic micrometastasis of colorectal cancer in nude rat.Methods:The model of colorectal cancer in nude rats were built by means of orthotopic transplantation with fresh neoplastic block into herniate cecum.Meanwhile,using Alu gene as a marker,hepatic micrometastasis of the nude rats were detected in different period after transplantation by means of PCR technique.Results:Most nude rats got cecal carcinoma 2 weeks after orthotopic transplantation with fresh neoplastic block into herniate cecum.Alu gene was detected in hepatic tissue in 57.1% of nude rats 4 weeks after transplantation in which hepatic metastases were not histopathologically detectable.Conclusion:Establishment of colorectal cancer model in nude rat by means of orthotopic transplantation with fresh neoplastic block into herniate cecum is simple and with high success rate.Micrometastases can be detected in liver in most nude rats 4 weeks after transplantation using PCR technique.