甘薯品种鉴定中指纹图谱的建立和应用

Establishment of DNA fingerprint and its application to variety identification in sweet potato

应用RAPD分析技术体系,构建了甘薯品种的指纹图谱.结果表明,在20μLPCR反应体积中,含有10mmol·L-1Tris-HCl(pH8.3)、50mmol·L-1KCl、2.5mmol·L-1MgCl2、100μmol·L-1dNTP、0.3μmol·L-1引物、40ng模板、1.5UTaq酶的反应体系适合甘薯RAPD分析.利用上述甘薯RAPD分析的指纹图谱,鉴定了10个供试品种.

RAPD analysis technical system for sweet potato was established, and successfully used in the construction of its DNA fingerprint. The results showed that this system in 20 μL reaction solution, which contained 10 mmol·L-1 TrisHCl (pH8.3), 50 mmol·L-1 KCl, 2.5 mmol·L-1 MgCl2, 100 μmol·L-1dNTP, 0.3 μmol·L-1 random primer, 40 ng DNA template, 1.5 U Taq DNA polymerase, was suitable for sweet potato. According to the DNA fingerprint, ten sweet potato cultivars were identified.