鸡马立克氏病发病过程中肝肾细胞凋亡的电镜观察

The electron microscopic observation on apoptosis of hepatocyte and renal tabular epithelial cells in the process of Marek′s disease of chickens

通过透射电镜对人工诱发的马立克氏病病鸡的肝、肾细胞进行了细胞凋亡观察,其肝、肾实质细胞发生了细胞凋亡.细胞凋亡呈现多阶段的过程:早期,染色质发生凝聚和边集;中期,染色质凝集十分明显,核变成致密小体,随后裂解成数个团块;后期,凋亡小体形成.肿瘤细胞浸润越严重,细胞凋亡也越明显.凋亡的细胞细胞质内自噬体和同心板层小体形成,内质网扩张成空泡状.细胞膜完整,线粒体无明显变化.

The apoptosis of hepatocyte and renal tabular epithelial cells of chickens infected with Marek′s disease virus (MDV) was observed with transmission electron microscope. The results showed that apoptosis could be seen in hepatocyte and renal tabular epithelial cells of the infected chickens. Under electron microscope, the apoptosis went through a multistaged process. At the early stage, the chromatin was condensed and marginated. At the intermediate stage, the chromatin condensation was very striking, the nucleus became condensed bodies. Then, it fragmented into serveral pieces. At the final stage, apototic bodies formed. The more serious the infiltration of tumor cells was, the more obvious the apoptosis in the tissue was. In the cytoplasm, the endoplasmic reticulum swelled and vacuolized, concentric laminar body and autophagosomes formed. The changes of mitochondrions were not significant. 