流动注射化学发光测定维生素B_6

Determination of Vitamin B_6 by Flow Injection Chemiluminescence Method

基于维生素B6在碱性条件下对Luminol和KIO4的化学发光反应有很强的增敏作用,结合反向流动注射技术,建立了流动注射化学发光测定维生素B6的新方法。在优化条件下,维生素B6浓度在5.0×10-8～1.0×10-5g/mL范围内,与化学发光强度呈良好的线性关系,方法的检出限为1.3×10-8g/mL,对5.0×10-6g/mL维生素B6进行11次平行测定,方法的相对标准偏差为1.5%。该方法成功用于药剂中维生素B6含量的测定。

A highly sensitive Chemiluminesence(CL) system for the determination of vitamin B6 with reverse flow-injection analysis is proposed based on the enhancement of CL reaction of KlO4 with Luminol. The relationship between the CL intensity and the concentration of vitamin B6 is linear in the range of 5.0×10-8～1.0×10-5 g/mL. The relative standard deviation is 1.5% (n=11,c=5.0×10-6 g/mL).The detection limit of penfluridol is 1.3×10-8 g/mL. The method has been applied to the determination of vitamin B6 in tablet and injections with satisfactory results.