碳纳米管修饰电极用于高效液相色谱对全血中巯基化合物的测定

Application for Multiwall Carbon Nanotubes Modified Electrode to Liquid Chromatography for Determination of the Thiols in Human Whole Blood

研究了羧基化多壁碳纳米管修饰电极 (MWNT CME)的制备方法和该修饰电极对巯基化合物的电催化行为 ,并首次以该修饰电极为电化学检测器 ,与高效液相色谱 (HPLC)联用 ,分离检测了半胱氨酸 (L Cys)和谷胱甘肽 (GSH)两种巯基化合物。结果表明在 3 .0× 1 0 - 7～ 1 .0× 1 0 - 3mol L浓度范围内 ,L Cys和GSH的浓度分别与其氧化峰的峰电流呈良好的线性关系 ,线性相关系数分别为 0 .9987和 0 .9990 ;检出限分别为 1 .2×1 0 - 7mol L和 2 .2× 1 0 - 7mol L。将该方法用于人全血中L Cys和GSH的测定 ,获得了满意的结果 ,为电分析化学在临床医学。

A new chemically modified electrode(CME) was fabricated based on the immobilization of multi-wall carbon nanotubes fuctionalized with carboxylic group. The results indicated that the CME exhibited efficiently electrocatalytic oxidation for L-cysteine and glutathione with relatively high sensitivity, stability and long-life. Coupled with high performance liquid chromatography, the CME is utilized for electrochemical detection of thiols. The peak currents of L-cysteine and glutathione were linear to their concentrations ranged from 3.0x10(-7) mol/L to 1.0x10(-3) mol/L with the calculated detection limits of 1.2x10(-7) mol/L, 2.2x10(-7) mol/L, respectively. The method had been successfully applied to assess the contents of L-cysteine and glutathione in human whole blood.