神经营养因子促进视神经夹伤后视网膜生长相关蛋白-43表达

Neurotrophic factors promoting the expression of retinal growth associated protein-43 in retina after crush injuries of the optic nerve

目的 观察大鼠视神经夹伤后视网膜的神经生长相关蛋白 - 43( growth associat-ed protein- 43,GAP- 43)的表达变化及睫状神经营养因子 ( ciliary neurotrophic factor,CNTF )和腺病毒介导脑源性神经营养因子 ( adenovirally delivered brain- derived neu-rotrophic factor,Ad- BDNF)对视神经夹伤的保护作用。方法 在眼球后 2 m m处作视神经夹伤 ,制作 SD大鼠视神经夹伤模型 ,通过巩膜进行玻璃体微量注射神经营养因子 ( neuro-traphic factors,NFs) ,应用免疫印迹法观察视网膜的 GAP- 43表达量的变化。结果 正常SD大鼠视网膜上 GAP- 43呈现低表达 ,分子量约为 46 .7ku;玻璃体注射 CNTF,大鼠视神经夹伤后 2周 ,GAP- 43的表达增强 ( P<0 .0 5 ) ;玻璃体注射 Ad- BDNF,在视神经夹伤后 4周内 GAP- 43的表达增强 ( P<0 .0 5 ) ,但这种作用以视神经损伤后 1周时最为明显。结论玻璃体注射 NFs能够在一定时间范围内促进视神经夹伤的 SD大鼠视网膜上 GAP- 43表达 ,其中 Ad- BDNF的促进作用能够维持相对较长的时间。

ObjectiveTo observe the growth associated protein-43 ( GAP-43 ) expression in the rat retina after injuries of the optic nerve, and the protection of the optic nerve with ciliary neurotrophic factor ( CNTF ) and adenovirally delivered brain-derived neurotrophic factor ( Ad-BDNF).MethodsThe optic nerve were crushed 2mm behind the eye ball of SD rats. The neurothophic factors ( NFs ) were intravitreal injected immediately after optic nerve crush. The expression of GAP-43 in the rat retina was observed by western-blot analysis. ResultsThere was low GAP-43 expression in normal SD rat retina, and the molecular weight was about 46.7ku. Intravitreal injection of CNTF after optic nerve crush could promote the expression of GAP-43 in rats retina in 2 weeks (P<0 05 ).Meanwhile intravitreal injection of Ad-BDNF after optic nerve crush could promote the expression of GAP-43 in rats retina in 4 weeks (P<0.05 ). But this promoting effect was more obvious 1 week after crush.ConclusionIntravitreal injection of NFs could promote the expression of GAP-43 in rat retina after optic nerve crush. But this effect is time-limited, and the Ad-BDNT could promote it for a longer time.