共聚焦显微镜观测血管紧张素Ⅱ对体外培养的牛眼小梁细胞纤维粘连蛋白的影响

Observation for the angiotensinⅡ influences on the fibronectin of cultured bovine trabecular meshwork cells with laser scanning confocal microscopy

目的 通过共聚焦显微镜观测血管紧张素 ( angiotensin ,Ang )对体外培养的牛眼小梁细胞纤维粘连蛋白 ( fibronectin)的影响来探讨 Ang 在青光眼发病机制中的意义。方法 体外培养的牛眼小梁细胞分别以 10 - 7、10 - 8mol· L- 1 Ang 以及受体拮抗剂孵育 2 4h,以间接免疫荧光法行纤维粘连蛋白染色后用共聚焦显微镜观测并定量分析。结果 10 - 7m ol· L- 1组和 10 - 8m ol· L- 1组纤维粘连蛋白荧光强度明显高于受体拮抗剂组和对照组。结论 Ang 在体外培养条件下可引起牛眼小梁细胞纤维粘连蛋白合成增加 ,可能导致细胞外基质在小梁网的堆积而引起房水引流阻力的增加 ,此效应可被 AT1 受体拮抗剂部分阻断。

ObjectiveTo observe the change of fibronectin effected by angiotensinⅡ(AngⅡ)in cultrued bovine trabecular meshwork cells(TM cells).Therefore may provide insight about Ang into the pathogensis of glaucoma.MethodsCultured bovine TM cells were incubated with AngⅡ(divided into 10 -7,10 -8mol·L -1group and AT 1 receptor antagonist Losartan pretreated group),stained with fibronectin by immunofluorecein methods after incubated 24h, observed and analysed with laser scanning confocal microscopy.ResultsFluorecein intensity of fibronectin in the 10 -7mol·L -1group and 10 -8mol·L -1group are stronger than control group and antagonist group.ConclusionAngⅡ can caused an increase in levels of fibronectin in bovine TM cells in vitro, may be result in accumulation of extracellular matrix and increases the resistance of aqueous outflow in TM. This effect can be partically inhibited by AT 1 receptor antagonist.