新生鼠缺氧缺血性脑损伤脑组织中趋化因子gro、MIP1-βmRNA的表达及意义

Study on the Expression and significance of mRNA for gro、MIP1-βin Cerebral Tissue of Newborn Rat With Hypoxic-ischemic Brain Damage

【目的】 观察新生鼠缺氧缺血性脑损伤 (HIBD)后脑组织中趋化因子生长相关基因 (gro)、巨噬细胞炎性蛋白 1 β(MIP1 β)mRNA表达的变化与炎症细胞反应的关系。 【方法】 采用 7d龄SD大鼠缺氧缺血性脑损伤模型 ,用逆转录 聚合酶链反应 (RT PCR)方法 ,动态观察脑组织中趋化因子gro、MIP1 βmRNA水平变化 ,同时制备石蜡切片H&E染色观察中性粒细胞、淋巴细胞反应 ,研究二者在时间上的关系。 【结果】 α 趋化因子 gromRNA的诱导先于缺血区中性粒细胞的浸润 ,β 趋化因子MIP1 βmRNA的表达先于淋巴细胞等的出现。 【结论】 趋化因子在HIBD免疫炎症细胞反应的激活过程中可能起重要作用。

To observe the expression of mRNA for the α chemokine growth related gene (gro) and the β chemokine macrophage inflammatory protein 1 β(MIP1 β) in cerebral tissue after hypoxic ischemic brain damage(HIBD) in newborn rat and study its relationship with inflammatory response. The dynamic changes of expression of mRNA for gro,MIP1 β in the cerebral tissue were studied by using 7d old SD rat hypoxic ischemic brain damage model and RT PCR technique.Meanwhile,the invasion of neutrophils and lymphocytes in the infarcted area were observed in the sections stained with H&E. Hypoxia ischemia rapidly induced mRNA for α chemokine gro which preceded the accumulation of neutrophils.Induction of β chemokine MIP1 β preceded the appearance of lymphocytes in the infarcted area. [Conclusions] These data suggests a critical role of chemokines for immunoinflammatory activation.