摘要
用Breuilacterium sp.DGCDC-82在7L发酵罐中分批培养。分别对添加剂吐温－80、培养温度、培养基的pH、通风量和搅拌速度 在胆固醇氧化酶的生产中的影响进行了研究。结果显示以上条件均对产酶有影响。在不同的发酵阶段改变发酵操作条件，发酵20h最高酶活可达1203U／L，生产强度可达60U／（L·h）。既有产地提高了胆固醇氧化酶的产量，又防止了发酵过程中的泡沫外溢。
Brevibacterium sp. was grown in a batch fermenter. The effect of Tween-80, cultivation temperature, pH of the culture medium, aeration rate and agitation speed on the production of choles-terol oxidase (COD)by the organism were examined. The results revealed that all of them effected the production of COD. Adjusting the operation variables could increase the production of COD effectively and prevent the occurrence of foam overflow during the fermentation. By adjusting operation condition, a maximum COD activity of 1 203 U/L with a volumetric production rate of 60 U/(L·h) per L could be achieved at 20 h.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2003年第2期34-37,43,共5页
Journal of Wuxi University of Light Industry
关键词
胆固醇氧化酶
短杆菌
发酵
cholesterol oxidase
Brevibacterium sp.
fermentation
