谷氨酰胺产生菌NS611的代谢流分析

The Metabolic Flux Analysis of Corynebacterium Glutamicum NS611

建立并完善了谷氨酸棒杆菌NS611生产谷氨酰胺的中心碳代谢网络.研究了菌体生长和生产阶段的代谢流分布,结果说明该菌葡萄糖的代谢以糖酵解途径为主,在产酸阶段丙酮酸羧化反应的代谢流量较大。NADPH不是谷氨酸棒杆菌NS611代谢的限制性因素.研究了氮饥饿处理对代谢流分布的影响,提出要提高谷氨酰胺的产量必须适当保留α-酮戊二酸的向下氧化的功能,以保证能量供给.

In this paper, a metabolic network of the Corynebacterium glutamicum NS611 was estab-lished and modified. It showed that glucose was mainly metabolized through the EMP pathway. In the production phase, the metabolic flux of the pyruvate carboxylation was large. NADPH was not the limiting factor of the metabolism of Corynebacterium glutamicum NS611. The metabolic fluxes of the Corynebacterium glutamicum NS611 with and without nitrogen starvation were compared. It showed that the oxidation of the a-ketoglutarate should be properly retained to ensure the energy supply.