从海鱼鱼白中提取核酸

Extraction of Nucleic Acid from Protamine in Sea Fish Processing

对地产海鱼加工下脚料鱼白中核酸含量测定、提取方法、产品纯度测定和组分鉴定方法进行了研究.结果显示在样品预处理过程中,低温抽提法比索氏抽提器法造成核酸损失要小.核酸粗产品收率为6.72％时,核酸总质量分数为2.30％.

The methods of detecting the nucleic acid content, extracting the nucleic acid from pro-tamine in sea fish processing, determinenating product purity, and identifing constituent have been studied in this paper. In the course of samples pro-processing, the low temperature extracting method has less nucleic acid loss than the Soxhlet' s methods. The yield of raw nucleic acid product from pro-tamine is 6.72 % , and the total nucleic acid content in the raw product is 2.30 % .