人脑神经干细胞的分离培养和鉴定

Isolation, Culture and Characterization of Neural Stem Cells from Human Brain

目的 探讨人脑神经干细胞的分离培养和鉴定方法。 方法 采用无血清培养液从人胚胎脑皮质分离和培养神经干细胞 ,并应用免疫荧光技术进行细胞自我更新能力、巢蛋白表达和多向分化潜能的鉴定。 结果 鉴定表明从人胚胎脑皮质分离培养的细胞具有神经干细胞的特征 ,并可在体外增殖 ,经诱导可分化为神经元、星型胶质细胞和少突胶质细胞。 结论 从人胚胎脑皮质成功分离培养了神经干细胞 ,它是研究细胞诱导分化的良好模型。

Objective\ To isolate, culture and characterize the neural stem cells from human brain.\ Methods\ Neural stem cells from human embryonic cerebral cortex were isolated and cultured using serum\|free medium.\ Their self\|renewal ability, expression of nestin and multipotent of differentiation was identified with immunofluorescence staining.\ Results\ Neural stem cells were isolated from human embryonic cerebral cortex by serum\|free medium and capable of proliferate and long\|term survival in vitro.\ They could differentiate into neurons, astrocytes and oligodendrocytes.\ Conclusion\ Neural stem cells are successfully cultured from human embryonic cerebral cortex.\;