棉铃虫对几种信息化合物的触角电位 (EAG)反应

Effects of interaction of plant volatiles and sex pheromone on EAG response of Helicoverpa armigera(Hüber)

通过棉铃虫成虫对 1 0种寄主植物挥发性物质、两种性信息素组分及两者的混合作用的触角电位反应 ( EAG) ,发现棉铃虫雌雄蛾对 1 0种挥发性物质的 EAG反应差异显著 ( P<0 .0 5 ) ,说明了 1 0种挥发物质对棉铃虫成虫的感应功能有所不同。 1 0种寄主植物挥发物质与性信息素主要组分混合后能引起雄蛾 EAG反应明显高于单独性信息素的反应 ,其中有 4种挥发性物质明显地增强棉铃虫对性信息素的反应 ( P<0 .0 5 ) ,即庚醛、1 -己醇、反 - 2 -己烯醇、顺 - 3-己醇 - 1 ,说明了以寄主植物挥发物质与昆虫性信息素混合作用来增强昆虫性信息素的应用效果。不同光温条件下饲养的雄蛾对寄主植物挥发性物质与性信息素相互作用的 EAG反应差异显著 ( P<0 .0 5 )。

The predilection data got from mixture of sex pheromone with infochemicals might reflex the real dynamics of insect population better than with insect sex pheromone alone.Many researches showed that mixture of plant volatiles with insect sex pheromone could significantly enhance the attractive effect for moths.In order to explore the seasonal variation of adults Helicoverpa armigera in response to infochemicals such as plant volatiles and female sex pheromone or their mixture in different generation,the electroantennograms (EAG) in response to ten plant volatiles,sex pheromone as well as their mixture of adults H.armigera reared under different temperature and photoperiods condition mimicked the conditions of the second and third generation were studied.The results showed that the EAG values of virgin female,mated female and male to ten plant volatiles were significant different (P<0.05).The EAG response values of virgin female was highest,then was the male,while the mated female was the lowest,but all of them have the highest response when induced by heptanal.This indicated that different component of plant volatiles might induced different behavior function.Significant differences of EAG response of male reared under different temperature and photoperiod condition (26.5℃,14.5h;28℃,14h) were induced between mixtures of plant volatiles with sex pheromone and sex pheromone alone.The EAG value of male reared under 26.5℃,14.5h condition were higher than those under 28℃,14h.The results analyzed by Duncan comparison showed that four plant volatiles components:heptanal,1-hexenol,Z-3-hexenol and 2-hexenol respectively,could significantly enhance the male response to sex pheromone alone.However,other two components:1-pinene and benzlbenhyde,could significantly inhibit the male response to sex pheromone.It implied that male EAG response to plant volatiles were different in generations,synergism of some plant volatiles with sex pheromone could be used for improving the lure effect of sex pheromone.Among which,mixture of two plant volatiles components(heptanal and l-hexenol) and sex pheromone main components could induced the highest male EAG values.It suggested that mixture of these two plant volatiles and sex pheromone can be used for the synergism of sex pheromone for improving the attractive effect in practice.