结核分支杆菌镜检、培养和PCR技术诊断结核病的临床评估

Clinical evaluate the diagnostic value of tuberculosis for detection of M.tuberculosis PCR,culture and microscopy assay

目的 评估结核分支杆菌PCR、培养、镜检对结核病诊断的临床效果。方法 对 1 2 1 7例临床可疑结核病人用PCR、培养和镜检三种方法的阳性检出率进行比较。结果 不同临床标本用PCR技术诊断结核病 ,其敏感性高于培养和涂片镜检 ,阳性检出率分别为 :88.7% ,43 .4% ,2 6.5 %。结论 通过三种方法比较分析 ,说明PCR技术是一种速度快、检出率高的检测结核分支杆菌的重要方法 。

Objective To study the clinical effect of M.tuberculosis examination by PCR,culture and microscopy assay.Methods 1?217 specimens of the clinical suspicious tuberculosis cases were compared with 3 kinds of methods (PCR,culture and microscopy) in diagnosis of tuberculosis.Results The positive rate of PCR?culture and smear by the different clinical specimens were 88.7%,43.4%,26.5% respectively.Conclusion By means of analyze of comparation with 3 kinds of methods,PCR is the fast and its positive examiniatorial rate is the highest.For diagnosis of tuberculosis,it is one of important methods in detection of M.tuberculosis .