新疆蒙古族肝炎患者的TTV检测、克隆与测序分析

Cloning and sequencing of TTV in Mongolian patients with hepatitis in Xinjiang

目的分析TTV在新疆蒙古族、汉族肝炎患者的部分基因序列,为新疆各民族中TTV感染的流行病学调查提供可靠的依据。方法根据AB008394全基因序列设计引物,建立巢式聚合酶链反应(Nested-PCR),检测新疆地区各民族肝炎患者262例血清中的TTV DNA,对其中的11例TTV阳性者进行了克隆测序。结果本组11例部分基本序列与AB008394相比较,其核苷酸序列的同源性10例均在90％以上,仅蒙古族为87.1％。结论本研究证实新疆地区的蒙古族中存在TTV感染。

Objective To clone and sequence TTV in patients with hepatitis of various nationalities in Xinjiang to provide reliable evidence for epidemical investigation of TTV infection in people of various nationalities in Xinjiang. Methods TTV DNA was detected in 262 patients with hepatitis by nested PCR. The PCR products of 11 TTV - positive samples were cloned and sequenced. Results Comparing with AB008394, the homology of TTV DNA sequence in the 11 samples was over 90%. However, its was only 87.1 % in Mongolian patients with hepatitis. Conclusions There is TTV infection in Mongolian people in Xinjiang.