苏云金芽孢杆菌大型质粒DNA的小量提取

Miniscale Preparation for Large Plasmid DNA from Bacillus thuringiensis

以苏云金芽孢杆菌库斯塔克亚种、猝倒亚种及以色列亚种为材料,介绍了低拷贝大型质粒DNA的小量提取方法,该法采用PEG6000进行质粒纯化,省略了苯酚和氯仿抽提过程。实验证明,该法结果稳定,提取的质粒DNA产量和质量均符合大多数分子生物学实验的要求。

Miniprep of low copy number and large plasmid was done from three subspecies of Bacillus thuringiensis.The plasmid was purified using PEG 6000 instead of phenol and chloroform.Experiments demonstrate that consequences from this protocol were stable,the quality and yield of plasmid DNA produced from it have met the standard for many molecular biology experiments.