动物基因敲除研究的现状与展望

Research Progress and Prospect of Animal Gene Targeting

基因打靶技术是建立在胚胎干细胞和同源重组技术之上,可对基因组进行定位修饰的实验方法,尤其可以在转基因动物的遗传性状修饰中起到巨大的作用。本文简述了转基因、体细胞克隆和基因打靶的研究历史,以及这些技术对转基因动物制备的影响和展望。

Established upon the embryo stem cell technique and homologous recombination,gene targeting has been widely used in the genome specific manipulation,especially applied in the genetic trait modification of transgenic animal.Our paper reviewed the history of transgenic animal,somatic cloning and gene targeting,which indicates the influence towards the current status and prospect of the transgenic animal production.