液相原位反转录PCR:一种研究基因原位表达的有效方法

In-Well in Situ RT-PCR:A High Throughput Method for Gene Expression Assay

介绍了液相原位反转录PCR(in wellinsituRT PCR)的操作程序、存在的问题及改良方法 ,最后探讨了原位反转录PCR在研究RNA转录、加工、编辑和多聚腺苷酸化等方面的应用。

The method of in-well in situ RT -PCR,together with its revisions,was introduced in this paper.In addition,suggestions were made about how to use this method to analyze RNA transcription,processing,editing and polyadenylation,etc. ;