摘要
利用SSR分子标记技术 ,对棉属A、D染色体组二倍体及四倍体代表棉种进行了遗传多样性分析。供试的10个二倍体代表棉种间遗传多态性丰富 ,分子聚类结果与Fryxell棉属分类结果相同。分子水平上进一步揭示出属于D染色体组的拟似棉与其他D染色体组棉种的相似系数最低 ,A、D染色体组间相似系数很高。该结果支持拟似棉是D染色体组最原始棉种 ,棉属不同染色体组是共同起源、单元进化的理论。利用栽培的异源四倍体棉种不太适于研究棉属A。
Genetic diversity analysis of diploid and allotetraploid cotton species was carried out using SSR molecular markers by selecting representative species having A and (or) D genome in Gossypium .Ten diploid cotton species in A and D genomes had high polymorphism and the molecular cluster was consistent with Gossypium classification previously reported by Fryxell.From molecular level,G.gossypioides belonging to D genome had low similarity matrix compared with other diploid D genome species.Diploid cotton species separately having A or D genome had their high similarity matrix.The research supported that G.gossypioides was the most original cotton species among all D genome species,different genomes in Gossypium had common origin and made evolution separately.To better understand genetic events that accompany allopolyploid formation,we add 2 cultivated allotetraploid cotton species in our research materials.However,the results showed that it was not appropriate to study evolution of A and D genome in Gossypium using cultivated allotetraploid cotton species.In order to solve the question,original arboreum,herbaceum and wild types of allotetraploid cotton species should be adopted.Further evolution research on cotton species based on the transcription level of cotton genome is being carried on.
基金
国家自然科学基金资助项目 (3 980 0 0 89)~~
