水杨酸作用下东北红豆杉细胞的二维凝胶电泳分析

2-Dimensional Gel Electrophoresis on Protein Patterns from Suspension Cultures of Taxus cuspidate Induced by SA

东北红豆杉悬浮培养体系中加入适量浓度的水杨酸 ,染色结果表明 ,水杨酸可以增加细胞膜通透性 ,并可诱导部分细胞发生核凝集或核碎裂。提取细胞染色体DNA进行琼脂糖凝胶电泳 ,发现染色体DNA发生了部分降解。应用蛋白质双向凝胶电泳技术 ,研究了水杨酸处理后东北红豆杉细胞发生应激反应过程中蛋白质的表达情况 ,分析了处理细胞与正常细胞的蛋白质组差异 ,发现在水杨酸处理 4 8h后的样品中有 7个蛋白质差异点 ,而且有6个蛋白点仅在对照中检测到。结果表明 ,外加水杨酸改变了东北红豆杉细胞的基因表达 ,抑制部分蛋白合成的同时也合成了部分新蛋白质 ,这些蛋白可能与水杨酸的作用有关。

The permeability of cell membrane was enhanced by exogenous SA in the culture of Taxus cuspidata Nuclei condense and fragments were observed in some cells by using fluorescent microscope, and the degraded chromosomal DNA was observed by using agarose gel electrophoresis The changes in soluble proteins of the suspension cultures of Taxus cuspidata induced by salicylic acids were analyzed by using two dimensional polyacrylamide gel electrophoresis Comparing with the control, seven new protein spots were found and six protein spots were not found in the cultures grown with SA at 48h The results obtained showed that SA could induce the expression of some special proteins that might be related with the action of SA in the suspension cultures of Taxus cuspidata