青霉LQ-7植酸酶产生条件研究

Studies on the fermentation conditions of phytase from Penicillium sp. LQ-7

筛选到一株植酸酶高产菌株 ,对其进行诱变并研究了该菌株产植酸酶的最适条件。优化产酶液体培养基组成为 :3%可溶性淀粉 ,0 5 %蛋白胨 ,0 5 %NH4NO3 ,0 0 5 %MgSO4·7H2 O ,0 0 5 %FeSO4·7H2 O ,0 0 0 1%MnSO4·4H2 O ;发酵培养基的起始pH为 6 5 ,接种量 4%的条件下 30℃ ,135r min培养 72h可获得较高的植酸酶产量。少量 (2 5mg)植酸盐的加入对植酸酶有促进作用 ,但过量 (10mg以上 )时则会产生抑制作用。

A Penicillium sp.which can produce phytase with high-activity has been screened and mutated by UV.The best phytase-producing medium is: 3% soluble starch,0.5% peptone,0.5% NH 4NO 3,0.05% MgSO 4·7H 2O,0.05% FeSO 4·7H 2O,0.001% MnSO 4·4H 2O.Primary pH of medium at 6.5,inoculum size at 4%(V/V),fermentation for 72 hours are beneficial to the yield of phytase.The adding of a small quantity of phytate can stimulative the produce of phytase,while excessive phytate will inhibit the reaction.