重组阳离子抗菌肽体外抗菌生物活性的初步研究

Anti-microbe activities in vivo of recombined cationic anti-microbial peptide in the transgenic strain Chjch1

天然抗菌肽是生物体自身产生的拮抗物质。本实验利用实验室构建的基因工程菌Chjch1诱导表达了阳离子抗菌肽。表达蛋白经Tricine SDSPAGE凝胶电泳分析确定了重组蛋白单体分子质量的正确性。经凝胶成像系统分析确定表达量为 2 9 6 7mg/L。摇瓶发酵菌体最大生物量达 2 0 8g/L。活性实验表明 ,重组蛋白发酵上清液对微黄色八叠球菌和大肠杆菌具有毒杀力 ,30 μL ,96h的发酵上清液对微黄色八叠球菌抑菌直径达 2 6cm ,对大肠杆菌抑菌直径为 2 36cm。

The natural anti microbial peptides are the anti microbial materials in vivo . The transgenic strain Chjch1 which was constructed in this work was fermented for the purpose of expression of the cationic anti microbial peptide analyzed by Tricine SDS PAGE. It proved the expressed peptide has the right molecular weight. The expressed quantity of peptide,which was up to 29 67?mg/L,was got by the gel analysis system. The max biomass was up to 20 8?g/L when it was fermented in the 250?mL conical flask. The anti microbial experiments in vitro proved that the recombinant protein could inhibite the growth of Sarcina Subflava Ravenel and Escherichia coli , and their diameters are 2 6?cm and 2 36?cm, respectively.