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基于邻苯二胺电聚合膜的抗体固定化方法研究 被引量：2

Studies on Antibody Immobilization Based on o-Phenylenediamine Electropolymerized Films for Immunosensors
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摘要 A novel method based on electropolymerized films(Eps) is proposed for immobilizing antibodies(antigens) through a polyelectrolyte mediated layer. The o phenylenediamine Eps are deposited on the surfaces of quartz crystal microbalance(QCM) with an electropolymerized method using o phenylenediamine as the precursor. After self assembling a polystyrenesulfonate(PSS) layer on the Eps, the immobilization of goat anti IgG antibody, as an example, is investigated. The effects of experimental conditions about PSS self assembly, IgG antibody immobilization and immunoreaction et al . on the performances of immunosensor were discussed respectively. The QCM immunosensor can be used to quantitatively determine NHIgG in the range of 1.67-196.08 μg/mL. Moreover, the PSS and protein layer can easily be removed by shifting the pH, making the immunosensor regenerable. A novel method based on electropolymerized films(Eps) is proposed for immobilizing antibodies(antigens) through a polyelectrolyte mediated layer. The o phenylenediamine Eps are deposited on the surfaces of quartz crystal microbalance(QCM) with an electropolymerized method using o phenylenediamine as the precursor. After self assembling a polystyrenesulfonate(PSS) layer on the Eps, the immobilization of goat anti IgG antibody, as an example, is investigated. The effects of experimental conditions about PSS self assembly, IgG antibody immobilization and immunoreaction et al . on the performances of immunosensor were discussed respectively. The QCM immunosensor can be used to quantitatively determine NHIgG in the range of 1.67-196.08 μg/mL. Moreover, the PSS and protein layer can easily be removed by shifting the pH, making the immunosensor regenerable.
作者 李继山 吴朝阳 王桦 沈国励 俞汝勤
机构地区 化学生物传感与计量学国家重点实验室
出处 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2003年第4期624-626,共3页 Chemical Journal of Chinese Universities
基金 国家自然科学基金 (批准号 :2 9975 0 0 6 2 0 0 75 0 0 6) 教育部博士点基金 (批准号 :2 0 0 10 5 32 0 0 8)资助
关键词 邻苯二胺 电聚合膜 抗体 固定化方法 自组装 压电免疫传感器 o Phenylenediamine electropolymerized films PSS polyanion film Self assembly Piezoelectric immunosensors
分类号 R446.6 [医药卫生—诊断学] TP212.3 [自动化与计算机技术—检测技术与自动化装置]
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参考文献6

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同被引文献11

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高等学校化学学报
2003年 第4期

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