摘要
A novel method based on electropolymerized films(Eps) is proposed for immobilizing antibodies(antigens) through a polyelectrolyte mediated layer. The o phenylenediamine Eps are deposited on the surfaces of quartz crystal microbalance(QCM) with an electropolymerized method using o phenylenediamine as the precursor. After self assembling a polystyrenesulfonate(PSS) layer on the Eps, the immobilization of goat anti IgG antibody, as an example, is investigated. The effects of experimental conditions about PSS self assembly, IgG antibody immobilization and immunoreaction et al . on the performances of immunosensor were discussed respectively. The QCM immunosensor can be used to quantitatively determine NHIgG in the range of 1.67-196.08 μg/mL. Moreover, the PSS and protein layer can easily be removed by shifting the pH, making the immunosensor regenerable.
A novel method based on electropolymerized films(Eps) is proposed for immobilizing antibodies(antigens) through a polyelectrolyte mediated layer. The o phenylenediamine Eps are deposited on the surfaces of quartz crystal microbalance(QCM) with an electropolymerized method using o phenylenediamine as the precursor. After self assembling a polystyrenesulfonate(PSS) layer on the Eps, the immobilization of goat anti IgG antibody, as an example, is investigated. The effects of experimental conditions about PSS self assembly, IgG antibody immobilization and immunoreaction et al . on the performances of immunosensor were discussed respectively. The QCM immunosensor can be used to quantitatively determine NHIgG in the range of 1.67-196.08 μg/mL. Moreover, the PSS and protein layer can easily be removed by shifting the pH, making the immunosensor regenerable.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2003年第4期624-626,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :2 9975 0 0 6
2 0 0 75 0 0 6)
教育部博士点基金 (批准号 :2 0 0 10 5 32 0 0 8)资助
关键词
邻苯二胺
电聚合膜
抗体
固定化方法
自组装
压电免疫传感器
o Phenylenediamine electropolymerized films
PSS polyanion film
Self assembly
Piezoelectric immunosensors