乳腺癌蛋白质组学研究

Proteomics in Breast Cancer

乳腺癌是妇女中最常见的一种癌症,鉴定出与乳腺癌癌变相关的蛋白质以及病情发展过程中蛋白质的变化对揭示乳腺癌变机理及早期诊断是非常重要的.早在蛋白质组学这一概念提出以前,人们已应用2 维凝胶电泳技术(2DE)研究乳腺癌的癌变机理,且随着人类基因序列测序的完成,质谱的应用,以及生物信息学的引入,蛋白质组的研究获得了飞速发展,高通量的蛋白质组研究以及新的技术如激光捕获显微切割(LCM),表面加强激光解吸 电离飞行时间质谱(SELDI TOF),蛋白质阵列,组织阵列等蛋白质组学技术已被用于乳腺癌研究并获得了很快速的发展.乳腺癌蛋白质组学研究已经鉴定了一些具有诊断潜能的生物分子靶标和信号传导因子.介绍了乳腺癌蛋白质组学研究中所使用的最新研究方法和研究进展.

Breast cancer is the most common form of cancer among women.The identification of proteins associated with breast cancer carcinogenesis,as well as the different stages of this pathalogy,is of critical importance.Twodimensional electrophoresis(2DE) has been used for studying breast cancer before the emergy of proteomics.However,with the completion of human genomic sequencing and the introduction of mass spectrometry,combined with bioinformatics for protein identification,breast cancer research has been considerably increased.The research of breast cancer will be developed more quickly by means of newer technologies such as laser capture microdissect(LCM),surfaceenhanced laser desorption/ionization timeof flight (SELDITOF),protein array and tissue array.A few of molecular markers have already identified by breast cancer proteomics research.In this paper,we simply introduce the newest technologies and some performance in breast cancer proteomics research.