依立雄胺对前列腺癌细胞(LNCaP)前列腺特异性抗原和Bcl-2蛋白体外表达的抑制作用

Inhibitory effect of epristeride on the expression of prostate specific antigen and Bcl-2 protein in human prostatic carcinoma LNCaP cells in vitro

目的 为研究依立雄胺治疗前列腺癌的可能性及可能机制 ,探讨依立雄胺体外对人激素依赖型前列腺癌细胞 (LNCaP)生长及前列腺特异性抗原(PSA)与Bcl 2蛋白表达的作用。方法 用 5 ,15和4 5 μmol·L- 1的依立雄胺作用LNCaP细胞 3d或 7d后 ,相差显微镜进行细胞形态学观察 ;台盼蓝染色活细胞计数 ,绘制 7d的生长曲线 ;应用流式细胞仪分析依立雄胺对LNCaP细胞凋亡的影响 ;应用免疫组化ABC比较经依立雄胺作用后LNCaP细胞PSA与Bcl 2蛋白的表达强度。结果 4 5 μmol·L- 1的依立雄胺作用LNCaP细胞 3d后 ,可使其明显的皱缩脱壁 ,部分细胞膜破裂。在 5 ,15和 4 5 μmol·L- 1的依立雄胺作用 7d后 ,可明显抑制LNCaP细胞的生长 ,抑制率分别达 4 0 .0 % ,4 7.4 %和 5 4 .3% ;流式细胞仪分析显示上述浓度的依立雄胺可诱导细胞凋亡 ,凋亡率分别为 3.5 % ,15 .8%和 2 5 .0 % ,而对照组为2 .2 % ;免疫组化分析表明依立雄胺可降低LNCaP细胞PSA和Bcl 2蛋白的表达。结论 在上述浓度下依立雄胺可特异性的抑制LNCaP细胞的生长和PSA与Bcl

AIM To investigate the possibility of treating prostate carcinoma with epristeride and its possible mechanisms. METHODS The prostate carcinoma LNCaP cells were treated with epristeride for 3 d or 7 d. Cell morphology was observed under phase-contrast microscope, cell viability was detected by trypan blue method. Apoptosis was detected by flow cytometer, the expressions of prostate specific antigen(PSA) and Bcl-2 protein were detected by immunohistochemical method. RESULTS Epristeride inhibited the growth of human prostatic cancer LNCaP cells with the inhibition rate of 40.0%, 47.4% and 54.3% when exposed to 5, 15 and 45 μmol·L -1 epristeride for 7 d, respectively. The epristeride in the three concentrations induced apoptosis in the LNCaP cells with the apoptosis rate of 3.5%, 15.8% and 25.0%, respectively, and the apoptosis rate in the control group was 2.2%. The epristeride dose-dependently decreased the expression of PSA and Bcl-2 protein in the LNCaP cells. CONCLUSION Epristeride is able to inhibit the growth and the expression of PSA and Bcl-2 protein of human prostate carcinoma cell line LNCaP, thus inducing apoptosis in LNCaP cells in the three concentrations in vitro.