长足大竹象交配行为

Mating behavior of Cyrtotrachelus buqueti( Coleoptera: Curculionidae)

【目的】探索长足大竹象Cyrtotrachelus buqueti Guerin-Meneville成虫的交配行为规律。【方法】通过室内饲养和野外观察相结合的方法,记录了完整的交配过程,比较了不同寄主及虫体大小间交配行为的差异。【结果】长足大竹象一次完整的交配包括示爱、抱对、插入输精和配后保护4个阶段,雌雄都有多次交配行为。室内试验证明,长足大足象完整交配时间为21.71±1.84 min,其中示爱时间为0.46±0.05 min,抱对时间为6.18±0.38 min,插入输精时间为8.36±0.62 min,配后保护时间为6.71±0.79 min。雄虫初次交配时,在示爱、抱对和插入输精时间上均显著长于有交配经历的雄虫(P<0.05)。长足大竹象在不同寄主植物上的交配时间存在差异,在慈竹上的示爱时间(0.46±0.05 min)和插入输精时间(8.36±0.62 min)显著长于在唐竹、撑×绿杂交竹、孝顺竹和芦竹上的时间(P<0.05);在孝顺竹上的配后保护时间显著长于在其他植物上的时间(P<0.05)。长足大竹象成虫会选择最适体长的配偶进行交配,当雌虫体长≥3.3 cm和3.0~3.2 cm时,与不同体长的雄虫在示爱、抱对、插入输精和配后保护时间上都显著长于其他体长的雌虫(P<0.05)。林间长足大竹象的交配行为与室内观察结果基本一致。【结论】研究结果有助于了解长足大竹象交配过程,也对研究该虫的繁殖行为学及行为控制技术提供了依据。

【Aim】This study aims to explore the mating behavior patterns for adults of the bamboo weevil,Cyrtotrachelus buqueti Guerin-Meneville. 【Methods】We recorded the whole mating process of C. buqueti adults by the combination of laboratory rearing with field observation and compared the differences in mating behaviors of C. buqueti adults on different hosts and with various body sizes. 【Results】The results showed that a complete mating of C. buqueti adults involves four phases,i. e.,courtship display,pairbonding,ejaculation and post-copulation guarding. There were multiple mating behaviors in the whole mating process of the female and male adults of C. buqueti. Laboratory observation showed that one copulation of C.buqueti lasted 21. 71 ± 1. 84 min,the courtship display lasted 0. 46 ± 0. 05 min,the pair-bonding lasted 6. 18 ±0. 38 min,and the ejaculation and post-copulation guarding lasted 8. 36 ± 0. 62 min and 6. 71 ± 0. 79 min,respectively. In virgin males,the time for courtship display,pair-bonding and ejaculations was significantly longer than that in mated males( P < 0. 05). The time for courtship display( 0. 46 ± 0. 05 min) and ejaculation( 8.36 ±0.62 min) of C. buqueti mated on Sinocalamus afris was significantly longer than that on Sinobambusa tootsik,Bambusa pervariabilisi × Dendrocalamopsis daii,Bambusa multiplex and Arundo donax,and there was the longest time of post-copulation guarding or placation on Bambusa multiplex( P < 0. 05). C. buqueti adults preferred to choose spouse in large body size for mating. When male body length was ≥3. 3 cm or 3. 0- 3. 2cm,the time of courtship display,pair-bonding,ejaculation and post-copulation guarding of males with different body lengths was longer than those of females( P < 0. 05). Mating behavior of C. buqueti observed in the field was basically consistent with the results observed in the laboratory. 【Conclusion】The results could help us understand the mating behavior of C. buqueti adults,and further provide scientific basis for theresearch of adult reproductive behaviors and behavior control technology of this insect.