共生菌Tremblaya princeps在扶桑绵粉蚧个体发育中的动态变化

Dynamics of Tremblaya princeps during individual development of its host Phenacoccus solenopsis( Hemiptera: Pseudococcidae)

【目的】通过研究共生菌Tremblaya princeps的形态、分布及动态变化,明确该菌在宿主扶桑绵粉蚧Phenacoccus solenopsis体内的基本生物学特性。【方法】通过透射电镜技术观察T.princeps的超微结构;利用实时定量PCR明确T.princeps的分布和数量变化。【结果】扶桑绵粉蚧体内T.princeps的分布因性别而异。在雌虫中,T.princeps集中分布于含菌体,在其他组织器官中无分布;含菌体的大小与雌虫各个阶段的个体大小呈显著线性相关,且临近成虫期,含菌体的膨大速率加快。在雄虫中,T.princeps在个体发育的不同阶段,数量变化明显,在蛹期数量急剧增加,之后相对减少;含菌体结构在预蛹期消解。【结论】共生菌T.princeps的变化因宿主性别而异,且与宿主个体发育存在明显的相关性。

【Aim】This study aims to explore the characteristics of the symbiotic bacterium Tremblaya princeps in Phenacoccus solenopsis through observing its morphology,location and dynamics in different stages of hosts. 【Methods 】 The ultrastructure of T. princeps was observed by transmission electron microscopy,and its location and quantitative changes were determined using real-time PCR. 【Results】T. princeps is located in different tissues compared between females and males of the host P. solenopsis.In females,T. princeps is located only in bacteriome,but not in any other tissues or organs of the host.Size of bacteriome had a significant linear relationship with host body size,and its expanding rate accelerated as the host adults developed. In males of the host,the density of T. princeps increased dramatically before the pupal stage,and then decreased as the adult emerged. Bacteriome collapsed when the males of the host entered the pre-pupal stage. 【Conclusion】Our results suggest that the infection status of T. princeps is varied with host sexuality and correlated to host individual development.