成人急性髓性白血病中p16/p15缺失与甲基化

Deletion and Methylation ofp16 / p15 Genes Analysis in Adult Acute Myeloid Leukemia

目的探讨p16/p15基因第二外显子在成人急性髓性白血病(AML)中的缺失与甲基化。方法 用常规PCR及酶切技术研究33例成人AML中p16/p15基因第二外显子的缺失与甲基化情况。结果 统计学分析p16 E2的HapII、SacII和NruI各位点之间以及p16 E2和p15 E2的HapII位点之间的甲基化状况无相关性(均为P>0.005),成人AML患者组与正常对照组p16 E2甲基化状况在。HapII位点无显著差异(P=0.078),在SacII和NruI位点有显著差异(P=0.037)。结论 成人AML中p16/p15基因第二外显子的缺失不是主要失活方式,基因编码区位点甲基化的发生可能是随机的,p16E2的甲基化在成人AML基因诊断中有一定作用。

Objective To discuss the deletion and methylation of p16 and p15 genes' E2 in adult acute myeloid leukemia( AML) . Methods Using regular enzyme PCR technques,deletions of p16 and p15 genes' E2 were detected in 33 patients with adult AML and methylation status of HapII,SacII and Nrul loci in p16 and p15 genes'E2 .Results Through statistical methylations of HapII,SacII and Nrul loci in p16 E2 no ratations were found respectively. Between adult AML patients and normal controls, methylations of HapII loci in p16 E2 were not quite different( P = 0.078) ;Methylation of SacII and Nrul loci in p16 E2 existed evident difference( P = 0.037) .Conclusions In adult AML patients, deletions of p16E2 and p15E2 are not the chief ways, the generations of locus methylation are random. Both genes'E2 methylations have certain significance of diagnosis for adult AML.