TTV在非甲至非庚型肝炎中感染的检测和分析

Detection of TTV in Non - A - G Hepatitis and Partial GeneSequence Analysis

目的研究新近报导的1种与输血后肝炎有关的病毒(TTV)在中国郑州地区非甲至非庚型肝炎患者中的感染和基因序列变异情况。方法采用TTV基因组ORF1区的套式聚合酶链反应(nested—PCR)方法对非甲至非庚型肝炎患者和正常人血清标本进行检测,并对非甲至非庚型肝炎患者中的TTV分离株进行序列测定。结果 TTVDNA在40例非甲至非庚型肝炎患者中检出18例,阳性率为45％,在96例正常人中检出17例,阳性率为17.7％,两组阳性率相比差异有统计学意义。TTV分离株序列与日本株(CLON22)相对应位置的核苷酸同源性为99.1％。结论 TTV在非甲至非庚型肝炎患者中的感染率较高,可能是非甲至非庚型肝炎的主要致病因子。TTV分离株与日本株可能属同一基因型。

Objective To investigate the prevalence of TTV infection in non - A - G hepatitis patients. Methods DNA of TTV was tested by polymerase chain reaction with nested primers in non - A - G hepatitis patients. Results DNA of TTV was detected 18(45% ) in the 40 patients with non - A - G hepatitis, and 17 (17.7%) in the 96 normal persons. The partial sequence of TTV isolates showed 99.1% nucleotide identity over the corresponding region of TTV in Japan (CLON22). Conclusions The results suggest that TTV would be the major cause of patients with non-A-G hepatitis.