麝香保心丸对鸡胚绒毛尿囊膜及培养的血管内皮细胞的促血管生成作用

Angiogenesis Promoting Effect of Shexiang Baoxin Pill on Chicken Embryo Chorioallantoic Membrane and Cultured Microvascular Endothelial Cells

目的 :探讨麝香保心丸在鸡胚绒毛尿囊膜模型及培养的微血管内皮细胞系统中的促血管生成作用。方法 :分别以重组碱性成纤维细胞生长因子 (bFGF ,贝复济 )和生理盐水为阳性和阴性对照 ,作用于绒毛尿囊膜 (chrioallantoicmembrane ,CAM) ,通过一级、二级血管计数 ,初步评价麝香保心丸的促血管生成活性 ;并通过观察它对培养的牛肾上腺微血管内皮细胞增殖及管腔结构形成的影响 ,验证其促血管生成效应 ;同时检测麝香保心丸作用于培养的人脐静脉内皮细胞血管内皮生长因子 (VEGF)和碱性成纤维细胞生长因子(bFGF)的mRNA表达及其上清液中VEGF、bFGF含量 ,初步探索其作用机理。结果 :麝香保心丸组CAM上血管数比生理盐水组明显增加 ;麝香保心丸组内皮细胞及管腔数目均较生理盐水组明显增多 ,前者细胞表达VEGF和bFGFmRNA明显增高 ,上清液中VEGF和bFGF含量明显增多。结论 :麝香保心丸能促进CAM及培养的微血管内皮细胞的血管生成。

Objective: To study the angiogenesis promoting effect of Shexiang Baoxin Pill (SXBXP) on chicken embryo chorioallantoic membrane (CAM) and cultured microvascular endothelial cells (MVEC). Methods: Using recombinant basic fibroblast growth factor (bFGF) and normal saline as positive and negative controls, the angiogenesis promoting effect of SXBXP on CAM was estimated primarily by counting the first and second grade vessels, which was then verified through observing the effect of SXBXP on the proliferation and tube formation of MVEC. The mechanism was studied by estimating vascular endothelial growth factor (VEGF), bFGF mRNA expression and their content in supernatant of cultured human umbilical vein endothelial cells (HUVEC). Results: Number of vessels in CAM and MVEC, and tube formation after SXBXP treatment were obviously more than those after treated with normal saline. Furthermore, VEGF and bFGF content and their mRNA expression in supernatant of HUVEC culture in the SXBXP group were higher than those in the control group. Conclusion: SXBXP could promote angiogenesis in CAM and MVEC.