MDV-gB重组痘苗病毒诱导的免疫保护性试验

Experiment on the detection of immunity and protection induced by MDV-gB recombinant vaccina virus

用MDV gB重组痘苗病毒RVV gB,HVT冻干苗、痘苗病毒WR株分别按试验程序,对细胞免疫及体液免疫检测试验中的1日龄SPF鸡进行免疫接种,并于15日龄对各组小鸡攻GA株强毒,后经过IFA检测抗体,以及以PHA为有丝分裂原检测淋巴C转化.结果表明,重组病毒和HVT冻干苗均获得较高保护,体液免疫差异不显著;细胞免疫在攻毒前差异不显著,但攻毒后第2d开始,RVV gB组淋巴细胞转化率明显高于HVT冻干苗.

Onedayold SPF chickens used in the detection of cellular and humoral immunity were inoculated with RVVgB,HVT freezedryingvaccine and vaccina virus respectively according to the procedure of experiment,and were challenged with GA virulent strain on 15th day Using IFA to detect antibody and PHA as mitogen to examine its lymphocyte transformation The results indicate that strong protection were acquired of both RVV and HVT freezedryingvaccine, the difference of humoral immunity is insignificant; the difference of cellular immunity is not significant either before challenge But two days challenge after,the lymphocyte of cransformation rate of RVVgB is obviously higher than that of HVT freezeddried groups