兔动脉粥样硬化过程中主动脉内膜水含量变化的研究

The Change of Water Content in Aortic Intima of Rabbit During Atherosclerosis

目的研究高脂饮食复制兔动脉粥样硬化过程中主动脉内膜水含量的变化。方法将45只新西兰大白兔随机分为普通饲料组(对照组)、高脂饲料组(高脂组)和高脂饲料加辛伐他汀组(辛伐他汀组),每组15只。在饲养的第8、16、24周末分别检测对照组、高脂组和辛伐他汀组血清中总胆固醇、甘油三酯和低密度脂蛋白胆固醇的浓度,HE染色观察主动脉的病理形态学变化并测量内膜厚度变化;将高脂组和辛伐他汀组主动脉弓内膜剥离,用真空冷冻干燥方法检测粥样硬化斑块内膜中水含量的变化。结果①高脂组血清中总胆固醇、甘油三酯和低密度脂蛋白胆固醇浓度均高于对照组和辛伐他汀组(P<0.05)。②对照组主动脉内膜薄而光滑;高脂组动脉内膜明显增厚,光镜下可见大量泡沫细胞和脂质斑块;辛伐他汀组内膜局限性增厚,斑块局限,泡沫细胞数目减少、体积变小。③高脂饮食诱导兔形成动脉粥样硬化过程中,高脂组16周、24周与8周相比粥样硬化斑块内膜中水含量明显减少,24周与16周相比也明显减少(P<0.05);辛伐他汀组16周、24周斑块内膜中水含量较高脂组相同时间点增多(P<0.05)。结论兔动脉粥样硬化形成过程中,随时间延长粥样硬化斑块内膜中水含量减少。

Aim To research the change of water content in the atherosclerotic rabbits' aortic intima during atherosclerosis at 8th,16th,24th week after high-cholesterol diet. Methods 45 New Zealand rabbits were divided into common control group,high-cholesterol diet group and simvastatin group randomly and equally. At the end of 8th,16th and 24th week,we detected the serum levels of total cholesterol( TC),triglyceride( TG) and low density lipoprotein cholesterol( LDLC) respectively. Aorta were stained with hematoxylin-eosin and were studied with optical microscope. The changes of thickness of aortic intimal were measured and aortic intima of aortic arch were stripped. The water content of atherosclerotic plaques was detected with the vacuum freeze drying. Results ①The serum levels of total cholesterol, triglyceride and low density lipoprotein cholesterol concentrations were higher in high-cholesterol diet group( P < 0. 05). ②Common control group: the intima was smooth and glossy; High-cholesterol diet group: the intima was thicker and a lot of foam cells and fatty streak plaques in the intima; Simvastatin group: thinner of intima and fewer foams cells and fatty steak plaques than high-cholesterol group. ③In the high-cholesterol diet group,the atherosclerosis was formed and the water content in 16th and 24th week group was lower than 8th week group( P < 0. 05). Compared with that of 16th week, the water content was reduced at the end of 24th week( P < 0. 05). In the simvastatin group,the water content was much more than the high-cholesterol diet group at the end of 16th and 24th week( P < 0. 05). Conclusion During atherosclerosis of rabbit,the water content was reduced in atherosclerotic plaques.