脉复康对高脂血症家兔血管内皮功能的影响

Effect of Maifukang on Endothelial Function in Rabbits with Hyperlipoidemia

目的 :研究活血化瘀中药脉复康对高脂家兔血管内皮功能的改善作用。方法 :实验分为 6组 :喂正常饲料 ;喂高脂饲料 (9周 ) ;喂高脂饲料 (5周 )并加喂脉复康Ⅰ号或Ⅱ号、Ⅲ号及绞股蓝总甙 (GPS、阳性中药 ) (4周 )。检查血清总胆固醇 (TC)、甘油三脂 (TG)、低密度脂蛋白 (LDL c)、高密度脂蛋白 (HDL c)、过氧化脂质 (LPO)、前列环素 (PGI2 )、血栓素A2 (TXA2 )的水平 ,以及用原位杂交法检查主动脉内膜内皮素基因 (ET mRNA)的表达。结果 :脉复康Ⅰ、Ⅱ、Ⅲ组与GPS组TC、TG、LDL c、TC HDL c、LPO、TXA2 、TXA2 PGI2 水平比高脂组明显降低。HDL c和PGI2 明显升高。ET mR NA过表达减弱。结论 :脉复康Ⅰ、Ⅱ、Ⅲ与GPS均具有改善血管内皮功能的作用 ,其中水蛭 (脉复康Ⅲ )的作用优于GPS。脉复康改善内皮功能以及逆转ET mRNA过表达 ,可能是活血化瘀的细胞与分子水平的机制。

Objective: To study the improved effect for Chinese medicine of promoting blood circulation and removing blood stasis(PBCRBS)-Maifukang on endothelial function in rabbits with Hyperlipoidemia. Methods: Rabbits were divided into 6 groups.ie,fed a standard diet; a high cholesterol diet(for 9 weeks);a high cholesterol diet(for 5 weeks)supplemented with the Maifukang Ⅰ or Ⅱ、Ⅲ and GPS(Gypenosides,Positive drug)(for 4 weeks).The levels of the serum total cholesterol(TC)、triglycerides(TG)、low density lipoprotein(LDL)、high density lipoprotein(HDL)、lipid peroxidation(LPO)、prostacyclin(PGI 2)、thtomboxane A 2(TXA 2)were detected.And the expression of endothelin gene (ET-mRNA)in aortic intima was observed by means of situ hydridization and histochemistry techniques.Results:In the Maifukang Ⅰ、Ⅱ、Ⅲand Gps-treated rabbits,TC、TG、LDL-C、TC/HDL、LPO、TXA 2、TXA 2/PGI 2 levels after 4 weeks were significantly lower than in the high cholesterol-fed rabbits.The HDL-c and PGI 2 levels increased significantly.The hyper-expression of ET-mRNA decreased.Conclusion:Maifukang Ⅰ or Ⅱ、Ⅲ and GPS could improve endothelial function in rabbits with hyperlipoidemia. But then the effects of Leech were superior to GPS. The observed effect of improving endothelial function and reversing hyper-expression of ET-mRNA may be the mechanism on cellular and molecular levels for Maifukang PBCRBS.