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NF-κB在黄芩甙诱导大鼠骨髓基质细胞分化为神经细胞中的作用(英文) 被引量:7

Role of NF-KB in Baicalin-Induced Differentiation of Rat Bone Marrow Stromal Cells into Neurons
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摘要 目的 研究核因子-κB(NF-κB)在中药单体黄芩甙体外诱导骨髓基质细胞分化为神经细胞的作用。方法 实验分为黄芩甙组和对照组。黄芩甙组采用黄芩甙作为主要诱导剂,诱导成年大鼠骨髓基质细胞6d。对照组仅用无血清培养基诱导。采用间接免疫荧光染色检测神经细胞标记蛋白和NF-κB亚基P65的表达。结果黄芩甙诱导6d,骨髓基质细胞形成较典型的神经细胞形态,同时表达多种神经细胞标记蛋白;对照组未见神经细胞标记蛋白表达。而且,对照组诱导6d,P65信号出现核移位,胞浆中信号明显减弱到(18.4±3.0)%,黄芩甙组P65信号(84.8±3.0)%主要仍在胞浆。结论 黄芩甙可以抑制NF-κB的活化,在骨髓基质细胞诱导分化为神经细胞过程中可能起到一定的作用。 [中国当代儿科杂志,2003,5(1):1—4] Objective To explore whether the transcription factor NF-####B takes part in baicalin-induced differentiation of bone marrow stromal cells (MSCs) into neurons. Methods MSCs from adult rats were induced by baicalin in the serum-free medium for 6 hs. The un-treated cells, as the control group, were only induced in the serum-free medium without baicalin. The expression of neuronal specific markers was evaluated by indirect immunofluorescence cytochemistry staining. The activity of NF-κB was measured by the presence of the NF-κB subunit RelA ( p65) translocated into the nucleus in the same way. Results After the induction by baicalin, MSCs displayed neuronal morphologies, such as pyramidal cell bodies and extensive networks of processes, and the expression of neuron-specific markers was detectable 6 ds after the induction. Neuronal specific marker proteins did not express in the control group. Six days after the induction, the P65 positive rate in the cytoplasm of the control group decreased to (18.4 ± 3.0) % , while in the baicalin group, the P65 positive rate in the cytoplasm was (84.8 ± 3.0)%. Conclusions Baicalin may inhibit the activation of NF-κB, which may act in the differentiation of MSCs into neurons. [Chin J Contemp Pediatr, 2003, 5(1): 1 - 4]
出处 《中国当代儿科杂志》 CAS CSCD 2003年第1期1-4,共4页 Chinese Journal of Contemporary Pediatrics
基金 National Natural Science Fundation(No.39870251) China Postdoctoral Science Fundation
关键词 黄芩甙 核因子-ΚB 骨髓基质细胞 神经细胞 Baicalin NF-κB Bone marrow stromal cells Neuron
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  • 1杨琴,贾延劼,杨军,董为伟,杨于嘉.核因子-κB在黄芩甙诱导大鼠骨髓基质细胞分化为神经细胞中的作用[J].中国中西医结合杂志,2005,25(3):248-251. 被引量:13
  • 2吕本强,邢学锋,罗佳波.注射用双黄连冻干粉化学成分的分离与鉴别[J].南方医科大学学报,2006,26(10):1471-1472. 被引量:11
  • 3Park KS, Lee YS, Kang KS. In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood. J Vet Sci 2006;7(4):343-348
  • 4Kang XQ, Zang WJ, Bao LJ, et al. Differentiating characterization of human umbilical cord blood-derived mesenchymal stem cells in vitro. Cell Biol Int 2006;30(7):569-575
  • 5Zhao MZ, Nonoguchi N, Ikeda N, et al. Novel therapeutic strategy for stroke in rats by bone marrow stromal cells and ex vivo HGF gene transfer with HSV-1 vector. J Cereb Blood Flow Metab 2006;26(9):1176-1188
  • 6Erices A, Conget P, Minguell JJ. Mesenchymal progenitor cells in human umbilical cord blood. Br J Haemato12000; 109(1 ): 235-242
  • 7Manca MF, Zwart I, Beo J, et al. Characterization of mesenchyrnal stromal cells derived from full-term umbilical cord blood. Cytotherapy 2008; 10(1):54-68
  • 8Lu LL, Liu Y J, Yang SG, et al. Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-suppordve function and other potentials. Haematologica 2006;91(8):1017-1026
  • 9Gutierrez H, Hale VA, Dolcet X, et al. NF-kappaB signalling regulates the growth of neural processes in the developing PNS and CNS. Development 2005; 132(7): 1713-1726
  • 10Mattson MP. NF-kappaB in the survival and plasticity of neurons. Neurochem Res 2005;30(6-7):883-893

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