腺嘌呤核苷酸转运体在压力负荷大鼠心肌组织中的表达

The expression of adenine nucleotide translocator in myocardium under pressure load in rats

目的 :研究心肌组织中腺嘌呤核苷酸转运体 (ANT)在高血压病心肌肥厚发生、发展中的作用。 方法 :采用腹主动脉缩窄法制作动物模型 ;心肌ANT1、ANT2mRNA的检测采用RT PCR计算机凝胶成像分析。 结果 :①手术组大鼠腹主动脉压、心重指数以及心肌病理切片均证实模型成功。②缩窄后约 4天ANT1mRNA的含量上调 ,而ANT2mRNA的含量下调 ,并于第 7天分别达到峰值和谷值。③术后第 14天手术组大鼠心肌ANT1mRNA和ANT2mRNA的含量均接近假手术组水平 ,并持续至实验结束。 结论 :ANT参与了高血压病心肌肥厚的发生、发展过程 ,且ANT1对这一过程中心肌细胞凋亡的调控占优势 。

Objectives: To study the role of myocardial adenine nucleotide translocator(ANT) during the occurrenc and development of hypertensive cardiac hypertrophy. Methods: The animal model of pressure load is made by means of abdominal aorta coarctation. The relative content of ANT1 mRNA and ANT2 mRNA in myocardium is detected by RT PCR and gel electrophoresis imaging. Results: ①Abdominal aorta pressure,cardiac weight index and myocardial pathological section of the operated group rats confirm the success of the animal model of pressure load. ②At about 4 days after coarctation, the level of ANT1 mRNA was up regulated but ANT2 mRNA was down regulated,and both reached the peak and valley respectively at 7 days. ③The levels of ANT1 mRNA and ANT2 mRNA are close to that of the sham operated group at 14 days after coarctation. Conclusions: Adenine nucleotide translocator(ANT) participate in the occurrence and development of hypertensive cardiac hypertrophy.And it may be ANT1 that is involved preferentially in the regulation of myocardial apoptosis,whereas the ANT2 might be more engaged in energy supply.