应用反相高效液相色谱法测定保健品中的腺苷

Determination of Adenosine in Functional Food by Reversed-phash High Performance Liquid Chromatographic Method

本文应用反相高效液相色谱线性梯度洗脱法对保健品中的腺苷进行分离腺苷测定。样品经充分粉碎后,准确称量(其中腺苷含量范围为0.25～0.1mg),加适量水,在超声波振荡器中萃取80min,经离心机离心,吸取上层清液,用0.45μm微孔滤膜过滤;用Lichrospher100 RP-C18柱,pH4.5～5.5的磷酸二氢钾溶液和甲醇作流动相,在1.0mL/min的流速和线性梯度洗脱下,能将腺苷完全分离,在波长260nm处,应用外标法定量。其标准曲线在(1～10)ug/mL的浓度范围内线性良好,其相关系数为1.000;6次重复变异系数为1.59％,加标回收率在103％～105％之间。

The adenosine in functional food was determined by reversed-phase high performance liquid chromatography and linearity gradient elution in this paper. The sample was pulverized fully, weighed accurately (adenosine content range 0.25-0. 1mg), solved with water, extracted 80min in ultrasonic disintegator, separated in centrifuge, filtrated by 0.45μm micropore filter. The adenosine was separated completely with Lichrospherl00 RP-C18 column. The mobile phase was sodium dihydrogen phosphate solution (pH = 4.5-5.5) and methanol. How rate is 1. 0mL/min in linearity gradient elution. Detection wavelenth is UV 260nm. Quantitative determination by external standard method. The calibration curves was linear in the range (1-10) μg / mL .The coefficients of determination was 1.000. The RSD was 1.59% .The recovery was range 103%-105%.