一氧化氮合酶在大鼠附睾内的表达及其可能的作用

The Expression of NOS in Epididymis of Male Rats and its Possible Role

目的 为了阐明一氧化氮合酶 (NOS)在附睾内的表达 ,同时探讨NO在男性生殖功能中的作用。方法 本研究采用正常大鼠附睾组织 ,用免疫组织化学ABC法研究两种NOS(iNOS、eNOS)的表达。结果 在附睾上皮细胞内eNOS及iNOS均为免疫反应阳性 ,其中eNOS表达强 ,iNOS表达较强 ,血管内皮及平滑肌细胞内仅为eNOS免疫反应阳性 ,附睾间质中仅iNOS为免疫反应阳性。结论 两种NOS在附睾内均有不同程度的表达 ,各有特定的分布区域 。

Objective To determine the expression of NOS in epididymis and explicate the effects of NO on the reproductive system of male rat.Methods Immunohistochemical ABC method was used to observe the localization of NOS. Results iNOS is distributed throughout the subepithelial tissue, eNOS and iNOS are expressed in the epididymal epithelial cells and only eNOS is expressed in smooth muscle cells of blood vessel. Conclusion iNOS and eNOS are all expressed in epididymis with the different distribution.