异质性细胞核核糖蛋白A2/B1在非小细胞肺癌的表达研究

Expression and its research of heterogeneous nuclear ribonucleoprotein A2/B1 in non-small cell lung cancer

目的观察异质性细胞核核糖蛋白A2/B1(HeterogeneousnuclearribonucleoproteinA2/B1,hnRNPA2/B1)在非小细胞肺癌的表达,探讨hnRNPA2/B1对非小细胞肺癌的诊断价值及与非小细胞肺癌临床分期、淋巴结转移的关系。方法采用免疫组织化学方法检测83例原发性非小细胞肺癌(肺癌组)、32例肺良性肿瘤(肺良性肿瘤组)及20例正常肺(正常肺组)组织中hnRNPA2/B1的表达。结果肺癌组hnRNPA2/B1表达阳性率79.50%(66/83),肺良性肿瘤组阳性率43.80%(14/32),正常肺组阳性率40.05%(8/20)。肺癌组阳性率显著高于肺良性肿瘤组及正常肺组(均P<0.05);肺良性肿瘤组阳性率与正常肺组无显著性差异(P>0.05)。Ⅰ～Ⅱ期肺癌的hnRNPA2/B1表达阳性率为67.60%(25/37),Ⅲ～Ⅳ期肺癌的阳性率为89.10%(41/46)。Ⅲ～Ⅳ期肺癌的阳性率显著高于Ⅰ～Ⅱ期肺癌(P<0.05)。有淋巴结转移患者的hnRNPA2/B1表达阳性率为88.00%(44/55),无淋巴结转移者的阳性率为66.70%(22/33)。有淋巴结转移患者的阳性率显著高于无淋巴结转移者(P<0.05)。结论hnRNPA2/B1过量表达与非小细胞肺癌的发生、发展和转移密切相关。检测hnRNPA2/B1对非小细胞肺癌的早期诊断、病情预测有重要的临床意义。

Objective To study the expression of hnRNP A2/B1 in patients with non-small cell lung cancer and its clinical significance. Methods The expression of hnRNP A2/B1 was measured by im-munohistochemical technique in 83 patients with non-small cell lung cancer,32 patients with lung benign tumor and 20 normal individuals. Results In 83 samples from patients with non-small cell lung cancer, hnRNP A2/B1 positivity rate was 79.50%(66/83).In 32 samples from patients with lung benign tumor, hnRNP A2/B1 positivity rate was 43.80%(14/32).In 20 samples from normal individuals, hnRNP A2/B1 positivity rate was 40.05%(8/20). The positivity rate of hnRNP A2/B1 was significantly higher in patients with non-small lung cancer than that in the other groups (P<0.05). In stage Ⅰ～Ⅱ, hnRNP A2/B1 positivity rate was 67.60%(25/37). In stage Ⅲ～Ⅳ, hnRNP A2/B1 positivity rate was 89.10%(41/46).The positivity rate of hnRNP A2/B1 in stage Ⅲ～Ⅳ was significantly higher than that in stage Ⅰ～Ⅱ(P<0.05). In patients without lymph nodes metastasis, hnRNP A2/B1 positivity rate was 66.70%(22/33). In patients with lymph nodes metastasis, hnRNP A2/B1 positivity rate was 88.00%(44/55).The positivity rate of hnRNP A2/B1 in patients with lymph nodes metastasis was significantly higher than that in patients without lymph nodes metastasis (P<0.05). Conclusion The overexpression of hnRNP A2/B1 is related to occurrence, progression and lymph nodes metastasis of non-small cell lung cancer. Thus this would be very important in early diagnosis and predicton of non-small cell lung cancer