摘要
To clone the full length cDNA of a gene responsible for vascular smooth muscle cell (v SMC) proliferation in atherogenesis, and study its function Methods Oxidized low density lipoprotein (ox LDL) at optimal concentration was used as the stimulant to induce v SMC proliferation in culture medium A cDNA subtractive library of v SMC proliferation specific to ox LDL stimulation was established using subtractive hybridization technique Methods, including blotting, Northern hybridization and gene sequencing, were used to clone new gene fragments By using full length cDNA screening and protein expression techniques, one full length cDNA was cloned and its function was studied Results One full length cDNA was cloned The new gene (Genbank AF 174647) expressed a 44 kDa protein, which might be associated with the activity of ox LDL Conclusion The new gene cloned may be associated with SMC proliferation in atherogenesis
To clone the full length cDNA of a gene responsible for vascular smooth muscle cell (v SMC) proliferation in atherogenesis, and study its function Methods Oxidized low density lipoprotein (ox LDL) at optimal concentration was used as the stimulant to induce v SMC proliferation in culture medium A cDNA subtractive library of v SMC proliferation specific to ox LDL stimulation was established using subtractive hybridization technique Methods, including blotting, Northern hybridization and gene sequencing, were used to clone new gene fragments By using full length cDNA screening and protein expression techniques, one full length cDNA was cloned and its function was studied Results One full length cDNA was cloned The new gene (Genbank AF 174647) expressed a 44 kDa protein, which might be associated with the activity of ox LDL Conclusion The new gene cloned may be associated with SMC proliferation in atherogenesis
基金
Thisworkwassupportedbythegrantof"scalingprogram"fromChineseMinistryofSciencesandTechnology (1 997- 2 0 0 0 )
