固相胰酶催化Leu^(B30)胰岛素的半合成

Enzymatjc Semisynthesis of Leu^(B30) Insulin by Immobilized Trypsin

用猪、牛等动物胰岛素对糖尿病患者做冶疗时,因这些胰岛素对人体是异体蛋白,在不同程度上具有一定的免疫反应性,长期服药会产生副作用。因此,近年来酶促半合成人胰岛素的研究受到了人们的重视。据报道,LeuB30胰岛素对人体具有最小的免疫反应性,且又有与人胰岛素相同的治疗作用。用固定化酶来进行LeuB30胰岛素的半合成至今未见报道。我们以猪胰岛素为原料,用固相胰酶催化半合成了LeuB30胰岛素。

Leu^(B30) insulin was semisynthesized by immobilized trypsin using porcine insulin as the starting material. The separation and characterization of the product was carried out by amino acid analysis and HPLC. The yield of Leu^(B30) insulin semisynthesized by immobilized trypsin is higher than that of insulin semisynthesized by native trypsin under the same conditions (60.5％ to 27.4％).