摘要
酿酒酵母 (Saccharomycescerevisiae)LCB1 (Longchainbase)基因被克隆到酵母诱导表达载体pYES2中 ,并转入到FY2中 ,用半乳糖诱导表达。与对照相比 ,质粒所含LCB1基因的表达 ,使酵母细胞干重略有下降 ,而神经酰胺的含量提高为对照的 1
LCB1 gene was cloned into inducible expression vector pYES2 and transformed into Saccharomyces cerevisiae FY2 The plasmid was induced to express with galactose. The cell dry weight of FY2 with plasmid LCB1 gene decreased a little, while ceramide contents increased to 1 9 times comparing to FY2 containing only pYES2
出处
《微生物学报》
CAS
CSCD
北大核心
2003年第2期189-193,共5页
Acta Microbiologica Sinica
基金
宝洁公司提供资助
关键词
酿酒酵母
LCB1基因
神经酰胺
表达
Saccharomyces cerevisiae, LCB1 gene, Ceramide, Expression
