TNP-470抑制小鼠恶性腹腔积液形成的实验研究

An experimental study of angiogenesis inhibitor TNP-470 on the formation of malignant ascites in mice

目的 :研究血管生成抑制剂TNP 4 70对小鼠恶性腹腔积液形成的抑制作用。方法 :将小鼠肝癌腹水型细胞系Hca F2 5接种于小鼠腹腔 (2× 10 5/鼠 )。将 10 0只小鼠随机分成对照组 (0 9%NS) ,化疗组 (DDP 1mg/kg) ,TNP 4 70低、中、高剂量组 (30mg/kg、6 0mg/kg、90mg/kg)。自第 2天起分别腹腔内给药 (0 2ml/鼠 ) ,隔日给药 ,共 7次。第 15天处死部分小鼠测各项指标 ,余小鼠观察生存期。结果 :化疗组 ,TNP 4 70低、中、高剂量组的腹水抑制率分别为 74 77% ,5 2 33%、80 11%和 92 89% ;腹膜瘤结节的形成率分别为 10 0 % ,70 %、80 %、5 0 %和 30 %。在生存时间上 ,各治疗组较对照组明显延长生存期 (P <0 0 5 )。结论 :TNP 4 70具有抑制小鼠恶性腹腔积液的形成和延长小鼠生存时间的作用。

Objective:The inhibition effect of angiogenesis inhibitor TNP 470 was investigated in a murine malignant ascites model.Methods:Hca F25 cancer cell (2×10 5/mouse) were inoculated in the murine peritoneal cavity. There are 100 mice that were randomized into 5 groups. From the 2 nd day, the control group was given 0 9% NS, the three treated groups were given 30 mg/kg, 60 mg/kg and 90 mg/kg of TNP 470 respectively, the chemotherapy group was given DDP 1 mg/kg. Each was injected i.p. every other day for 7 times. On the 15 th day, half of them were killed and examined.Results:The inhibition rates of malignant ascites of the DDP group and the three treated groups were 74 77%,52 33%,80 11% and 92 89% respectively. The rates of tumor nodes on peritoneum were 100%, 70%, 80%, 50% and 30% respectively. All the treated groups were longer than the control group on the survival rates.Conclusion:These results suggest that the angiogenesis inhibitor TNP 470 has a strong inhibitory effect on the formation of malignant ascites.