超临界CO_2连续浓缩鱼油EPA和DHA的研究

Continuous concentration of EPA and DHA from fish oil by supercritical CO 2

为了克服鱼油有效组分超临界 CO2 间歇萃取方法的缺点 ,设计建造了超临界 CO2 在内径 14 mm、填料高 1.8m的填料塔中连续萃取浓缩鱼油有效组分的流程。根据单因素试验的结果 ,对工艺参数进行优化。设计了 4因素 3水平并考虑部分交互作用的正交试验方案 ,以综合指标进行评价 ,得到了优化工艺参数为 :填料塔压为 12 .5 MPa;CO2 流量为 5L/m in;鱼油进料流量为 0 .8m L/m in;塔的温度分布为 40～ 85℃。经方差分析得知 ,温度分布的影响对综合指标高度显著 ,鱼油进料流量对综合指标的影响显著 ,试验范围内的压力、CO2 流量、压力和温度分布交互作用、温度分布和 CO2 流量交互作用以及压力和 CO2 流量交互作用对综合指标没有显著影响。

In order to overcome the disadvantages of a batch flow, a continuous flow was designed to concentrate EPA and DHA from fish oil by supercritical CO 2 extraction technology in packed tower with a diameter of 14 mm and a padding height of 1.8 m. Then, on the basis of single fact experiments, the technological parameters were optimized by orthogonal tests which include 3 factors and 4 levels, with the consideration of interaction among 3 factors, in which a comprehensive index was used as a test target. The results showed that the optimum column pressure was 12.5 MPa, temperature distribution was 40～85℃, CO 2 flow 5 L/min, and fish oil feed flow was 0.8 mL/min. It was found that the temperature distribution factor was very prominent, followed by the factor of fish oil feed flow. The effects of pressure and CO 2 flow, as well as the interactive effects among pressure and temperature distribution, temperature distribution and CO 2 flow, and pressure and CO 2 flow were not prominent.