淫羊藿甙对MC3T3-E1细胞活性及功能作用的实验研究

The experiment study of the effects of icariin on the activity in MC3T3-E1 cell in vitro

目的研究淫羊藿甙(ICA)对体外培养的成骨样细胞MC3T3-E1的活性及功能作用的影响,探讨ICA对成骨细胞生长、分化的作用。方法体外培养的MC3T3-E1细胞按ICA刺激浓度0、1×10-9、1×10-8及1×10-7mol/L分为4组,给药后24、48及72 h通过MTT法分别检测细胞活性;检测4、7、10 d的ALP活性以及7、14、21 d的骨钙素含量。结果在3个时间点,ICA组的OD值、ALP活性和OC含量均高于对照组;48、72 h时1×10-8组和1×10-9组的OD值均显著高于对照组(P<0.05);7 d的1×10-8组ALP、10 d的1×10-8组和1×10-9组ALP活性均显著高于对照组(P<0.05);14、21 d的1×10-8组和1×10-9组的降钙素含量均显著高于对照组(P<0.05)。结论 ICA对MC3T3-E1细胞的活性和成骨作用具有促进作用,其中以浓度为1×10-8mol/L效果最好。

Objective To explore the effect of icariin on the activity in preosteoblast MC3T3-E1 cells in vitro. Methods MC3T3-E1 cells were divided into four groups according to stimulus concentrations of icariin: 0、1 × 10- 9、1 × 10- 8、1 × 10- 7mol / L groups. After adding icariin,the activities of cells were tested using MTT method at 24 h、48 h and 72 h. It continued culture for 2-3weeks; alkaline phosphatase activity and osteocalcin were measured. Results After induced with icariin,MC3T3-E1 cells had higher OD value、ALP activity and OC value in 1 × 10- 8and 1 × 10- 9groups than the control one( P < 0. 05). Conclusions Icariin can improve the activity and osteogenesis,especially at the concentration of 1 × 10- 8mol / L.