基质金属蛋白酶-3在舍格伦综合征患者唾液和唇腺中的表达

Expression of matrix metalloproteinase-3 in salivary and labial gland tissue in patients with Sj?rgen syndrome

目的研究基质金属蛋白酶-3(matrix metalloproteinase-3,MMP-3)在舍格伦综合征患者唇腺和唾液中的表达水平,以及明确其表达水平与病理分级的关系。方法采集35例舍格伦综合征患者(实验组)和20例黏液腺囊肿患者(对照组)的唇腺和唾液,应用酶联免疫吸附试验法(ELISA)检测唾液中MMP-3的表达水平,应用免疫组化方法观察唇腺中MMP-3的表达,使用图像分析软件(IPP 6.0)定量分析两组唇腺中MMP-3的表达强度,并将舍格伦综合征患者按照其病理分级分为低的1、2级和高的3、4级两组。结果 MMP-3在实验组唾液和唇腺中的表达高于对照组,差异有统计学意义(P<0.05);MMP-3在病理分级高的患者唾液和唇腺中表达水平高于病理分级低的患者,差异有统计学意义(P<0.05)。结论舍格伦综合征患者唇腺中的MMP-3可能参与了腺泡以及腺体基质的破坏,导致疾病的发生;唾液以及唇腺中MMP-3的表达水平可能成为舍格伦综合征的一项检测指标,并为临床诊治提供参考依据。

Objective To investigate the expression of matrix metalloproteinase 3(MMP-3)in the labial glands and saliva of patients with Sjogren’s syndrome and to determine whether its expression level is correlated with pathological grade.Methods The glandular glands and saliva of 35 patients with Sjogren’s syndrome(experimental group)and 20 patients with mucinous gland cysts(control group)were collected.The enzyme-linked immunosorbent assay(ELISA)was used to detect the expression level of MMP-3 in the saliva of the two groups.Immunohistochemical method was used to observe the expression of MMP-3 in labial gland.The intensity of MMP-3 expression in the labial glands was quantified by using image analysis software(IPP 6.0).According to the pathological grading,patients with Sjogren syndrome were divided into two groups:low grades,grade 1 and grade 2,and high grades,grade 3 and grade 4.Results The expression of MMP-3 in saliva and labial glands of the experimental group was higher than that of the control group,and the difference was statistically significant(P<0.05).The expression level of MMP-3 in the saliva and labial glands of patients with high pathological grades was higher than that of low grades,and there was a statistically significant difference(P<0.05).Conclusion MMP-3 in the labial glands of patients with Sjogren’s syndrome may be involved in the destruction of acinar and glandular stroma,leading to the occurrence of disease.The expression level of MMP-3 in saliva and labial glands may become an indicator of Sjogren’s syndrome and provide a reference for clinical diagnosis and treatment.