壳聚糖基温敏凝胶膜体内引导骨再生性能研究

The study on properties of guided bone regeneration of new type chitosan-based thermosensitive hydrogel membranes in vivo

目的:观察壳聚糖/β-甘油磷酸钠(CS/β-GP,chitosan/β-glycerophosphate salt)温敏凝胶复合膜及其负载釉基质蛋白(EMPs,enamel matrix proteins)体内引导骨再生的生物特性。方法:体外合成CS/β-GP温敏凝胶复合膜并负载EMPs。10只健康成年雄性Wistar大鼠随机分为A、B两组,分别为实验1个月和2个月组。大鼠颅骨中线两侧制备直径5mm的极量骨缺损(critical size defect, CSD);左侧为实验侧,覆盖CS/β-GP (1.0g)复合膜负载EMPs,右侧覆盖单纯CS/β-GP复合膜为对照侧。通过大体观察、X线新生骨密度测量、HE染色等方法比较两组复合膜修复骨缺损的情况。结果:测量1个月、2个月双侧骨密度百分比,差异具有统计学意义(P<0.05);HE染色发现,术后1个月双侧均有少量新骨生成;术后2个月,实验侧内的新骨生成增加且更致密、趋于成熟。结论:该种类CS/β-GP复合膜具有引导骨再生的特性,负载生物活性因子后能明显加速骨愈合。

To observe chitosan/β-glycerophosphate salt(CS/β-GP)thermosensitive hydrogel membranes loaded enamel matrix proteins and detect the guided bone regeneration properties.Methods:CS/β-GP membranes were synthesized in vitro and loaded with EMPs.10 male and healthy Wistar rats were randomly divided into two groups(A and B).Critical size defect(CSD)in a diameter of 5mm was created on both sides of the skull of the rats.The experimental side on the left was covered by CS/β-GP(1.0g)composite membrane loaded EMPs on the left and the control side was covered by CS/β-GP(1.0g)membrane on the right.The bone reparation of the groups on both sides was compared by observation,density measurement of newly formed bone and hematoxylin-eosin staining.Results:The percentage of bilateral bone density measured at 1 month and 2 months had statistical significance(P<0.05).HE staining showed that there were a few new bone formation on both sides at 1 month after operation.At 2 months after operation,the new bone formation in the experimental side increased and became more compact and mature.Conclusion:This kind of CS/β-GP composite membrane has the characteristics of guided bone regeneration,and can significantly accelerate bone healing after loading bioactive factors.