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肝细胞生长因子与糖尿病大鼠早期肾小球肥大

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摘要 目的:检测肝细胞生长因子(HGF)和p^21WAF1/CIPI在早期糖尿病大鼠肾小球中的表达,探讨HGF在肾小球肥大中的作用。方法:免疫组化法检测糖尿病大鼠早期(第3,7,14d)HGF的蛋白和P21蛋白的表达,同时用酶联免疫吸附法(ELISA)法和反转录PCR(RT-PCR)检测了肾小球内HGF蛋白和mRNA水平。结果:HGF在早期糖尿病大鼠的表达主要位于肾小于内,HGF蛋白和mRNA均在第3d开始升高,并于第7d达到高峰,肾小球内P21蛋白的表达亦于第7d达到高峰,糖尿病组肾小球内HGF蛋白水平和P21蛋白积分呈正相关(r=0.9346,P<0.01),结论:糖尿病早期肾小球内有HGF的表达,其表达可能与糖尿病早期肾小球肥大有关。
出处 《临床肾脏病杂志》 2003年第1期22-24,F003,共4页 Journal Of Clinical Nephrology
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