基质溶素与早期大肠癌发生发展的相关性

Raletionship to the Expression of Matrilysin and theProgression of Early Colorectal Carcinoma

目的 :探讨基质溶素 (matrilysin ,MMP 7)与早期大肠癌发生、发展的关系。方法 :用逆转录聚合酶链式反应 (RT PCR)检测早期大肠癌和进展期大肠癌、正常肠粘膜、大肠腺瘤、变性隐窝病灶 (aberrantcryptfoci,ACF)中MMP 7mRNA的表达 ;用免疫组织化学SP法检测早期大肠癌癌灶周围肿瘤细胞MMP 7蛋白的表达。结果 :90 %(2 7 30 )的早期大肠癌MMP 7mRNA阳性表达 ,而相应正常粘膜几乎不表达 ;4 5例ACF只有 7例MMP 7mRNA弱阳性表达 ,10例腺瘤、2 0例进展期大肠癌均阳性表达。由正常肠粘膜、ACF、腺瘤、早期大肠癌到进展期大肠癌MMP 7mRNA表达水平逐渐增高 (P <0 .0 1)。 4 6 .7% (14 30 )早期大肠癌癌灶周围肿瘤细胞MMP 7蛋白阳性表达。早期大肠癌MMP 7的表达与肿瘤侵袭深度相关 (P <0 .0 5 ) ,有转移者MMP 7水平高于无转移者 (P <0 .0 5 )。结论 :MMP 7的高表达可能在早期大肠癌的发生。

Objective: To investigate the correlation of MMP 7 mRNA expression with the progression of early colorectal carcinoma. Methods: RT PCR technique was used to measure the expression of MMP 7 mRNA in clinical specimens, and matrilysin protein of early colorectal cancers at the invasive front was immunohistochenically analyzed. Results: MMP 7mRNA was observed in 90% (27/30) early colorectal carcinoma while it was almost undetectable in normal mucosas. MMP 7 mRNA was observed in 7 case of 45 ACF and observed. In all case of 10 colorectal adenomas and 20 advanced colorectal cancers. The level of MMP 7 mRNA gradually increased from normal muscosas, ACF,adenomas, early colorectal cancers to advanced colorectal cancers (P<0.01). MMP 7 protein was observed immunostainingly in more than 30%of cancer cells at the invasive front of 46%(14/30) early colorectal cancers. The level of MMP 7 increased in parallel with the depth of invasion (P<0.05). The level of MMP 7 in cancers with metastasis was higher than that with no metastasis(P<0.05). Conclusion: The over expression of MMP 7 may play an important role in infiltration and metastasis of early colorectal carcinoma.