抗神经生长因子抗体对SD大鼠股骨发育的作用

Developmental effect on rat long bone by systemic injection of anti-NGF antibody

目的:研究周围神经系统发育和调节功能对骨发育的影响。方法:将抗神经生长因子抗体经腹腔注射到新生SD大鼠体内,抑制周围神经系统的发育和调节功能,2d注射1次,持续两周,处理前后称量体重,处死大鼠后取全长股骨和坐骨神经。坐骨神经行Loyez髓鞘染色观察,股骨进行发育情况大体测量,并行生物力学检测。结果:药物处理对大鼠生理状态和运动能力无明显影响,处理前后处理组与对照组体重比较差异无显著性意义(P>0.05);处理组股骨冠状面中段皮质直径较对照组大(P<0.05),中径/长度比值也是处理组较大(P<0.05);生物力学检测处理组和对照组最大压应力和脆性比较均差异无显著性意义(P>0.05);坐骨神经轴突密度比较,两组差异无显著性意义。结论:采用抗神经生工因子(NGF)抗体抑制周围神经系统发育和调节功能,处理组大鼠下肢长骨的外径发育较快,但所承受最大压应力并无明显提高,说明骨皮质相对较薄,此外应力曲线斜率接近说明矿物/有机物比 例接近,提示该变化可能是发育过程中破骨细胞的功能相对活跃,导致成骨细胞代偿性成骨增多。

AIM: To study the effects of the peripheral nerve system on long bone development. METHODS: Anti-NGF antibody was injected systemically into newborn SD rats through enterocoelia. The rat weights were recorded everyday. After two weeks, the rats were killed and the sciatic nerves and the unilateral femurs were isolated. The thigh bones were investigated by both gross measurement and biomechanics investigation, and the developmental condition of sciatic nerves were taken pathological observation. RESULTS: Generally the living condition and weights of rats of treatment group was not much changed compared with control group ( P > 0. 05). The femur diameters and diameter/length of treatment group were higher than those of control group ( P < 0. 05) . But the difference of biomechanical perfonnance between two groups was not statistically significant ( P > 0.05) and the sciatic nerve of control group has more axons than that of treatment group. CONCLUSION: Long bone development is regulated by peripheral nerve system to some extent. The bigger outside diameter of femurs and the similar biomechanical properties to control group suggests the inside diameters of treatment group is bigger than those of control group. We make a assumption that during development the os-teoclast activity is relatively higher, and the cortex growth leading by os-teoblast is induced faster.